Application Note : 013
Title of application :
Detection of Ketamine as a Drug of Abuse from urine sample
Area of application :
Drug analysis/Biomedical analysis
Conditions :
| Buffer | KET-01 (20 mM), pH= 5.0; |
| Sample | Urine sample containing 1 mg/L ketamine; |
| Capillary | 50 mm i.d, 70 cm length; |
| Injection mode | Hydrodynamic injection; |
| Separation setting | + 20 kV |
| Instrument used | CE-L1, UV detection at 210 nm |
Description :
Detection of ketamine as an illicit drug from body fluids was commonly
conducted using GC, GC/MS, LC or LC/MS. Apart from involving bulky and costly
instrumentation, chromatographic analysis may also be complicated by the presence of
interference due to the endogenous components in the sample matrix. In contrast, under the
given CZE separation condition, ketamine was measured as the only component ahead of EOF,
with all the endogenous compouds migrating near EOF because of their electrical
neutrality. The excellent separation power, coupled with its operation simplicity, make
CE-L1 a preferred alternative in the determination of ketamine and other illicit drugs. It
is especially useful for drug regulatory agencies and law enforcement bodies as a routine
means of drug screening.
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