Application Note : 016
Title of application :
Analysis of cations of electrolyte in serum
Area of application :
Clinical analysis
Conditions :
| Buffer | CECATC1S |
| Sample | Human serum : 1. K+ 2. Na2+ 3. Ca2+ 4. Mg2+ 5 and 6. Unknowns |
| Capillary | 50 mm i.d, 40 cm length; |
| Injection mode | Hydrodynamic injection |
| Separation setting | + 8kV |
| Instrument used | CE-P1 (Potential gradient detection) |
Description :
Analysis of electrolytes in serum is very frequently required in clinical laboratories
because of their function of maintaining the osmotic pressure, distribution of water,
stability of the acid-base balance. This application note shows capillary
electrophoresis with potential gradient detection to be very useful in analysis of cations
of electrolytes in serum samples. The analytical method is simple and quick. No
sample preparation other than dilution is required before sample injection. Simultaneous
analysis of these electrolytes can provide comprehensive information on their
concentration levels for clinical uses. Instrumental setup cost and operation cost are low
comparing with other methods.
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